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Structure paper

TitleCryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
Journal, issue, pagesSci Rep, Vol. 10, Issue 1, Page 16301, Year 2020
Publish dateOct 1, 2020
AuthorsEileen L Murphy / Kavindra V Singh / Bryant Avila / Torsten Kleffmann / Steven T Gregory / Barbara E Murray / Kurt L Krause / Reza Khayat / Gerwald Jogl /
PubMed AbstractEnterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ...Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are similar to other bacterial ribosomes. Comparison of intersubunit conformations among five classes obtained after three-dimensional classification identifies several rotated states. Large ribosomal subunit protein bL31, which forms intersubunit bridges to the small ribosomal subunit, assumes different conformations in the five classes, revealing how contacts to the small subunit are maintained throughout intersubunit rotation. A tRNA observed in one of the five classes is positioned in a chimeric pe/E position in a rotated ribosomal state. The 70S ribosome structure of E. faecalis now extends our knowledge of bacterial ribosome structures and may serve as a basis for the development of novel antibiotic compounds effective against this pathogen.
External linksSci Rep / PubMed:33004869 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 4.14 Å
Structure data

0656

6o8w

EMDB-0656, PDB-6o8w:
Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class01
Method: EM (single particle) / Resolution: 3.53 Å

0657

6o8x

EMDB-0657, PDB-6o8x:
Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class02
Method: EM (single particle) / Resolution: 3.68 Å

0658

6o8y

EMDB-0658, PDB-6o8y:
Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class03
Method: EM (single particle) / Resolution: 4.14 Å

0659

6o8z

EMDB-0659, PDB-6o8z:
Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class04
Method: EM (single particle) / Resolution: 3.49 Å

0660

6o90

EMDB-0660, PDB-6o90:
Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class05
Method: EM (single particle) / Resolution: 3.49 Å

21562

6w6p

EMDB-21562, PDB-6w6p:
MultiBody Refinement of 70S Ribosome from Enterococcus faecalis
Method: EM (single particle) / Resolution: 2.9 Å

21907

6wu9

EMDB-21907, PDB-6wu9:
50S subunit of 70S Ribosome Enterococcus faecalis MultiBody refinement
Method: EM (single particle) / Resolution: 2.9 Å

21908

6wua

EMDB-21908, PDB-6wua:
30S subunit (head) of 70S Ribosome Enterococcus faecalis MultiBody refinement
Method: EM (single particle) / Resolution: 3.2 Å

21909

6wub

EMDB-21909, PDB-6wub:
30S subunit (head) of 70S Ribosome Enterococcus faecalis MultiBody refinement
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • enterococcus faecalis (bacteria)
  • enterococcus faecalis og1rf (bacteria)
KeywordsRIBOSOME / 70S / pathogen / antibiotic development / antibiotic resistant

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