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TitleStructures of the human spliceosomes before and after release of the ligated exon.
Journal, issue, pagesCell Res, Vol. 29, Issue 4, Page 274-285, Year 2019
Publish dateFeb 6, 2019
AuthorsXiaofeng Zhang / Xiechao Zhan / Chuangye Yan / Wenyu Zhang / Dongliang Liu / Jianlin Lei / Yigong Shi /
PubMed AbstractPre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ...Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ligation, have been structurally characterized. In this study, we report the cryo-electron microscopy structures of the human post-catalytic spliceosome (P complex) and intron lariat spliceosome (ILS) at average resolutions of 3.0 and 2.9 Å, respectively. In the P complex, the ligated exon remains anchored to loop I of U5 small nuclear RNA, and the 3'-splice site is recognized by the junction between the 5'-splice site and the branch point sequence. The ATPase/helicase Prp22, along with the ligated exon and eight other proteins, are dissociated in the P-to-ILS transition. Intriguingly, the ILS complex exists in two distinct conformations, one with the ATPase/helicase Prp43 and one without. Comparison of these three late-stage human spliceosomes reveals mechanistic insights into exon release and spliceosome disassembly.
External linksCell Res / PubMed:30728453 / PubMed Central
MethodsEM (single particle)
Resolution2.86 - 3.0 Å
Structure data

EMDB-9645, PDB-6icz:
Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-9646, PDB-6id0:
Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1 complex) at 2.9 angstrom resolution
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-9647, PDB-6id1:
Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex) at 2.9 angstrom resolution
Method: EM (single particle) / Resolution: 2.86 Å

Chemicals

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
  • Human (human)
KeywordsSPLICING / Human Post-catalytic Spliceosome / Human Intron Lariat Spliceosome

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