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Structure paper

TitleArchitecture of the CBF3-centromere complex of the budding yeast kinetochore.
Journal, issue, pagesNat Struct Mol Biol, Vol. 25, Issue 12, Page 1103-1110, Year 2018
Publish dateNov 26, 2018
AuthorsKaige Yan / Ziguo Zhang / Jing Yang / Stephen H McLaughlin / David Barford /
PubMed AbstractKinetochores are multicomponent complexes responsible for coordinating the attachment of centromeric DNA to mitotic-spindle microtubules. The point centromeres of budding yeast are organized into ...Kinetochores are multicomponent complexes responsible for coordinating the attachment of centromeric DNA to mitotic-spindle microtubules. The point centromeres of budding yeast are organized into three centromeric determining elements (CDEs), and are associated with the centromere-specific nucleosome Cse4. Deposition of Cse4 at CEN loci is dependent on the CBF3 complex that engages CDEIII to direct Cse4 nucleosomes to CDEII. To understand how CBF3 recognizes CDEIII and positions Cse4, we determined a cryo-EM structure of a CBF3-CEN complex. CBF3 interacts with CEN DNA as a head-to-head dimer that includes the whole of CDEIII and immediate 3' regions. Specific CEN-binding of CBF3 is mediated by a Cep3 subunit of one of the CBF3 protomers that forms major groove interactions with the conserved and essential CCG and TGT motifs of CDEIII. We propose a model for a CBF3-Cse4-CEN complex with implications for understanding CBF3-directed deposition of the Cse4 nucleosome at CEN loci.
External linksNat Struct Mol Biol / PubMed:30478265 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 8.5 Å
Structure data

0095

6gyp

EMDB-0095, PDB-6gyp:
Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore
Method: EM (single particle) / Resolution: 3.6 Å

0096

6gys

EMDB-0096, PDB-6gys:
Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore
Method: EM (single particle) / Resolution: 4.4 Å

0097

6gyu

EMDB-0097, PDB-6gyu:
Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-11626:
Structure of inner kinetochore CCAN-Cenp-A complex (Cenp-HIKTW)
Method: EM (single particle) / Resolution: 8.5 Å

Chemicals

ChemComp-MET:
METHIONINE

ChemComp-PHE:
PHENYLALANINE

ChemComp-ASN:
ASPARAGINE

ChemComp-ARG:
ARGININE

ChemComp-THR:
THREONINE

ChemComp-GLN:
GLUTAMINE

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsCELL CYCLE / Complex / DNA BINDING PROTEIN

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