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Structure paper

TitleStructure of a prehandover mammalian ribosomal SRP·SRP receptor targeting complex.
Journal, issue, pagesScience, Vol. 360, Issue 6386, Page 323-327, Year 2018
Publish dateApr 20, 2018
AuthorsKan Kobayashi / Ahmad Jomaa / Jae Ho Lee / Sowmya Chandrasekar / Daniel Boehringer / Shu-Ou Shan / Nenad Ban /
PubMed AbstractSignal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER ...Signal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER membrane followed by the transfer of the signal sequence to the translocon. Here, we present the cryo-electron microscopy structure of the mammalian translating ribosome in complex with SRP and SR in a conformation preceding signal sequence handover. The structure visualizes all eukaryotic-specific SRP and SR proteins and reveals their roles in stabilizing this conformation by forming a large protein assembly at the distal site of SRP RNA. We provide biochemical evidence that the guanosine triphosphate hydrolysis of SRP·SR is delayed at this stage, possibly to provide a time window for signal sequence handover to the translocon.
External linksScience / PubMed:29567807 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

4300

6frk

EMDB-4300, PDB-6frk:
Structure of a prehandover mammalian ribosomal SRP and SRP receptor targeting complex
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Source
  • oryctolagus cuniculus (rabbit)
  • canis lupus familiaris (dog)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLATION / ER membrane targeting ribosome Signal recognition particle

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