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Structure paper

TitleA fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin.
Journal, issue, pagesNat Commun, Vol. 9, Page 2106-2106, Year 2018
Publish dateJan 18, 2018 (structure data deposition date)
AuthorsMenchon, G. / Prota, A.E. / Lucena-Agell, D. / Bucher, P. / Jansen, R. / Irschik, H. / Muller, R. / Paterson, I. / Diaz, J.F. / Altmann, K.H. / Steinmetz, M.O.
External linksNat Commun / PubMed:29844393
MethodsX-ray diffraction
Resolution2.1 - 2.405 Å
Structure data

PDB-6fii:
Tubulin-Spongistatin complex
Method: X-RAY DIFFRACTION / Resolution: 2.405 Å

PDB-6fjf:
Tubulin-FcMaytansine complex
Method: X-RAY DIFFRACTION / Resolution: 2.402 Å

PDB-6fjm:
tubulin-Disorazole Z complex
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-SG9:
Spongistatin-1

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-DKE:
FcMaytansine

ChemComp-DKN:
Disorazole Z

Source
  • rattus norvegicus (Norway rat)
  • gallus gallus (chicken)
  • bos taurus (cattle)
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE

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