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TitleCrystallographic Trapping of Reaction Intermediates in Quinolinic Acid Synthesis by NadA.
Journal, issue, pagesACS Chem. Biol., Vol. 13, Page 1209-1217, Year 2018
Publish dateNov 29, 2017 (structure data deposition date)
AuthorsVolbeda, A. / Saez Cabodevilla, J. / Darnault, C. / Gigarel, O. / Han, T.H. / Renoux, O. / Hamelin, O. / Ollagnier-de-Choudens, S. / Amara, P. / Fontecilla-Camps, J.C.
External linksACS Chem. Biol. / PubMed:29641168
MethodsX-ray diffraction
Resolution1.5 - 2.3 Å
Structure data

PDB-6f48:
Structure of quinolinate synthase with reaction intermediates X and Y
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6f4d:
Structure of the Y21F variant of quinolinate synthase in complex with PGH
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6f4l:
Structure of quinolinate synthase with inhibitor-derived quinolinate
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6g74:
Structure of the Y21F variant of quinolinate synthase in complex with phthalate
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-XQB:
2-imino,3-carboxy,5-hydroxy,6-oxo hexanoic acid

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-YQA:
5-hydroxy,-4,5-dihydroquinolinate

ChemComp-HOH:
WATER

ChemComp-PGH:
PHOSPHOGLYCOLOHYDROXAMIC ACID

ChemComp-PO4:
PHOSPHATE ION

ChemComp-NTM:
QUINOLINIC ACID

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM

ChemComp-PHT:
PHTHALIC ACID

Source
  • thermotoga maritima (bacteria)
  • thermotoga maritima (strain atcc 43589 / msb8 / dsm 3109 / jcm 10099) (bacteria)
KeywordsTRANSFERASE / NAD BIOSYNTHESIS / IRON SULFUR CLUSTER

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