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Structure paper

TitleInsights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis.
Journal, issue, pagesNat Commun, Vol. 9, Page 3998-3998, Year 2018
Publish dateNov 27, 2017 (structure data deposition date)
AuthorsDorival, J. / Risser, F. / Jacob, C. / Collin, S. / Drager, G. / Paris, C. / Chagot, B. / Kirschning, A. / Gruez, A. / Weissman, K.J.
External linksNat Commun / PubMed:30266997
MethodsX-ray diffraction
Resolution2.5 - 3.15 Å
Structure data

PDB-6f32:
Crystal structure of a dual function amine oxidase/cyclase in complex with substrate analogues
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6f7l:
Crystal structure of LkcE R326Q mutant in complex with its substrate
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6f7v:
Crystal structure of LkcE E64Q mutant in complex with LC-KA05
Method: X-RAY DIFFRACTION / Resolution: 3.03 Å

PDB-6fjh:
Crystal structure of the seleniated LkcE from Streptomyces rochei
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-ACT:
ACETATE ION

ChemComp-CJ8:
(2~{R},3~{R})-2,3-bis(oxidanyl)-~{N},~{N}'-dipropyl-butanediamide

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-CJE:
ethyl (2~{R})-2-methyl-3-oxidanylidene-butanoate

ChemComp-HOH:
WATER

ChemComp-CWH:
[(2~{S},5~{R},8~{S},11~{S})-1-[(2~{R},3~{R},5~{R},6~{S})-3,5-dimethyl-6-oxidanyl-4-oxidanylidene-oxan-2-yl]-5,11-dimeth

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-NA:
Unknown entry

Source
  • streptomyces rochei (bacteria)
  • streptomyces rochei subsp. volubilis (bacteria)
KeywordsFLAVOPROTEIN / Amine oxydase / cyclase / Post-PKS enzyme / tayloring enzyme

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