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TitleAdenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 450, Year 2018
Publish dateJan 31, 2018
AuthorsMarkus Schmid / Patrick Ernst / Annemarie Honegger / Maarit Suomalainen / Martina Zimmermann / Lukas Braun / Sarah Stauffer / Cristian Thom / Birgit Dreier / Matthias Eibauer / Anja Kipar / Viola Vogel / Urs F Greber / Ohad Medalia / Andreas Plückthun /
PubMed AbstractMost systemic viral gene therapies have been limited by sequestration and degradation of virions, innate and adaptive immunity, and silencing of therapeutic genes within the target cells. Here we ...Most systemic viral gene therapies have been limited by sequestration and degradation of virions, innate and adaptive immunity, and silencing of therapeutic genes within the target cells. Here we engineer a high-affinity protein coat, shielding the most commonly used vector in clinical gene therapy, human adenovirus type 5. Using electron microscopy and crystallography we demonstrate a massive coverage of the virion surface through the hexon-shielding scFv fragment, trimerized to exploit the hexon symmetry and gain avidity. The shield reduces virion clearance in the liver. When the shielded particles are equipped with adaptor proteins, the virions deliver their payload genes into human cancer cells expressing HER2 or EGFR. The combination of shield and adapter also increases viral gene delivery to xenografted tumors in vivo, reduces liver off-targeting and immune neutralization. Our study highlights the power of protein engineering for viral vectors overcoming the challenges of local and systemic viral gene therapies.
External linksNat Commun / PubMed:29386504 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.8 - 7.4 Å
Structure data

3821

6eqc

EMDB-3821: Cryo-EM reconstruction of a modified human Adenovirus C5
PDB-6eqc: Cryo-EM reconstruction of a complex of a binding protein and human adenovirus C5 hexon
Method: EM (single particle) / Resolution: 7.4 Å

PDB-5ogi:
Complex of a binding protein and human adenovirus C 5 hexon
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-DIO:
1,4-DIETHYLENE DIOXIDE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
  • human adenovirus c serotype 5
  • human adenovirus 5
KeywordsVIRAL PROTEIN / Antibody / Human Adenovirus C5 / gene therapy / gene therapy Viral Protein

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