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TitleArchitecture of eukaryotic mRNA 3'-end processing machinery.
Journal, issue, pagesScience, Vol. 358, Issue 6366, Page 1056-1059, Year 2017
Publish dateNov 24, 2017
AuthorsAna Casañal / Ananthanarayanan Kumar / Chris H Hill / Ashley D Easter / Paul Emsley / Gianluca Degliesposti / Yuliya Gordiyenko / Balaji Santhanam / Jana Wolf / Katrin Wiederhold / Gillian L Dornan / Mark Skehel / Carol V Robinson / Lori A Passmore /
PubMed AbstractNewly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, ...Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the ~200-kilodalton polymerase module. This revealed four β propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3'-end processing.
External linksScience / PubMed:29074584 / PubMed Central
MethodsEM (single particle)
Resolution3.55 Å
Structure data

3908

6eoj

EMDB-3908, PDB-6eoj:
PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae
Method: EM (single particle) / Resolution: 3.55 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • SaccharSaccharomyces cerevisiae (strain ATCC 204508 / S288c)omyces (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsRNA BINDING PROTEIN / WD40 / Beta-propeller / Zinc finger / 3'end processing

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