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Structure paper

TitleBinding and Action of Amino Acid Analogs of Chloramphenicol upon the Bacterial Ribosome.
Journal, issue, pagesJ. Mol. Biol., Vol. 430, Page 842-852, Year 2018
Publish dateFeb 15, 2018 (structure data deposition date)
AuthorsTereshchenkov, A.G. / Dobosz-Bartoszek, M. / Osterman, I.A. / Marks, J. / Sergeeva, V.A. / Kasatsky, P. / Komarova, E.S. / Stavrianidi, A.N. / Rodin, I.A. / Konevega, A.L. ...Tereshchenkov, A.G. / Dobosz-Bartoszek, M. / Osterman, I.A. / Marks, J. / Sergeeva, V.A. / Kasatsky, P. / Komarova, E.S. / Stavrianidi, A.N. / Rodin, I.A. / Konevega, A.L. / Sergiev, P.V. / Sumbatyan, N.V. / Mankin, A.S. / Bogdanov, A.A. / Polikanov, Y.S.
External linksJ. Mol. Biol. / PubMed:29410130
MethodsX-ray diffraction
Resolution2.6 - 2.8 Å
Structure data

PDB-6cfj:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6cfk:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6cfl:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-EZG:
N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-EZP:
N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-ARG:
ARGININE

ChemComp-EZM:
N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide

Source
  • escherichia coli (E. coli)
  • salmonella enterica subsp. enterica serovar gallinarum str. 287/91 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • enterobacteria phage t4 (virus)
  • escherichia coli (strain k12) (bacteria)
Keywordsribosome/inhibitor / Chloramphenicol / antibiotic / inhibitor / ribosome / 70S / ribosome structure / inhibition of translation / peptidyl transferase center / ribosome-inhibitor complex

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