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TitleHepatitis B virus core protein allosteric modulators can distort and disrupt intact capsids.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateJan 29, 2018
AuthorsChristopher John Schlicksup / Joseph Che-Yen Wang / Samson Francis / Balasubramanian Venkatakrishnan / William W Turner / Michael VanNieuwenhze / Adam Zlotnick /
PubMed AbstractDefining mechanisms of direct-acting antivirals facilitates drug development and our understanding of virus function. Heteroaryldihydropyrimidines (HAPs) inappropriately activate assembly of ...Defining mechanisms of direct-acting antivirals facilitates drug development and our understanding of virus function. Heteroaryldihydropyrimidines (HAPs) inappropriately activate assembly of hepatitis B virus (HBV) core protein (Cp), suppressing formation of virions. We examined a fluorophore-labeled HAP, HAP-TAMRA. HAP-TAMRA induced Cp assembly and also bound pre-assembled capsids. Kinetic and spectroscopic studies imply that HAP-binding sites are usually not available but are bound cooperatively. Using cryo-EM, we observed that HAP-TAMRA asymmetrically deformed capsids, creating a heterogeneous array of sharp angles, flat regions, and outright breaks. To achieve high resolution reconstruction (<4 Å), we introduced a disulfide crosslink that rescued particle symmetry. We deduced that HAP-TAMRA caused quasi-sixfold vertices to become flatter and fivefold more angular. This transition led to asymmetric faceting. That a disordered crosslink could rescue symmetry implies that capsids have tensegrity properties. Capsid distortion and disruption is a new mechanism by which molecules like the HAPs can block HBV infection.
External linksElife / PubMed:29377794 / PubMed Central
MethodsEM (single particle)
Resolution4.0 Å
Structure data

7294

6bvf

EMDB-7294, PDB-6bvf:
Cryo-EM Structure of Hepatitis B virus T=4 capsid in complex with the fluorescent allosteric modulator HAP-TAMRA
Method: EM (single particle) / Resolution: 4.0 Å

7295

6bvn

EMDB-7295, PDB-6bvn:
Cryo-EM Structure of Hepatitis B virus T=3 capsid in complex with the fluorescent allosteric modulator HAP-TAMRA
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-E9D:
Heteroaryldihydropyrimidine tetramethylrodamine

Source
  • hepatitis b virus genotype d subtype adw
KeywordsVIRUS LIKE PARTICLE / capsid / CpAM / antiviral / fluorescent

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