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TitleSolution Structures of Engineered Vault Particles.
Journal, issue, pagesStructure, Vol. 26, Issue 4, Page 619-626.e3, Year 2018
Publish dateApr 3, 2018
AuthorsKe Ding / Xing Zhang / Jan Mrazek / Valerie A Kickhoefer / Mason Lai / Hwee L Ng / Otto O Yang / Leonard H Rome / Z Hong Zhou /
PubMed AbstractPrior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat ...Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution (∼4.8 Å) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer has three regions: body, shoulder, and cap. While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 Å, resulting in an outward-projected cap. Our structures clarify the structural discrepancies in the body region in the prior crystallography models. The vault's drug-delivery potential is highlighted by the internal disposition and structural flexibility of its Gag-loaded N-terminal extension at the barrel waist of the engineered vault.
External linksStructure / PubMed:29551289 / PubMed Central
MethodsEM (single particle)
Resolution4.9 Å
Structure data

EMDB-7125, PDB-6bp7:
Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 2
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-7126, PDB-6bp8:
Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 1
Method: EM (single particle) / Resolution: 4.9 Å

Source
  • rattus norvegicus (Norway rat)
KeywordsSTRUCTURAL PROTEIN / Vault Recombinant protein structure Engineered nano-particle

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