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Structure paper

TitleThe microenvironment surrounding FAD mediates its conversion to 8-formyl-FAD in Aspergillus oryzae RIB40 formate oxidase.
Journal, issue, pagesJ. Biochem., Vol. 166, Page 67-75, Year 2019
Publish dateMay 6, 2018 (structure data deposition date)
AuthorsDoubayashi, D. / Oki, M. / Mikami, B. / Uchida, H.
External linksJ. Biochem. / PubMed:30715389
MethodsX-ray diffraction
Resolution2.4 - 2.441 Å
Structure data

PDB-5zu2:
Effect of mutation (R554A) on FAD modification in Aspergillus oryzae RIB40formate oxidase
Method: X-RAY DIFFRACTION / Resolution: 2.441 Å

PDB-5zu3:
Effect of mutation (R554K) on FAD modification in Aspergillus oryzae RIB40formate oxidase
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-FAY:
[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl

Source
  • aspergillus oryzae (strain atcc 42149 / rib 40) (mold)
KeywordsOXIDOREDUCTASE / Formate oxidase / 8-formyl-FAD / effect of mutation

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