Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 115, Issue 17, Page 4423-4428, Year 2018
Publish date	Apr 24, 2018
Authors	Xiong Pi / Lirong Tian / Huai-En Dai / Xiaochun Qin / Lingpeng Cheng / Tingyun Kuang / Sen-Fang Sui / Jian-Ren Shen /
PubMed Abstract	Photosystem I (PSI) is one of the two photosystems present in oxygenic photosynthetic organisms and functions to harvest and convert light energy into chemical energy in photosynthesis. In eukaryotic ...Photosystem I (PSI) is one of the two photosystems present in oxygenic photosynthetic organisms and functions to harvest and convert light energy into chemical energy in photosynthesis. In eukaryotic algae and higher plants, PSI consists of a core surrounded by variable species and numbers of light-harvesting complex (LHC)I proteins, forming a PSI-LHCI supercomplex. Here, we report cryo-EM structures of PSI-LHCR from the red alga in two forms, one with three Lhcr subunits attached to the side, similar to that of higher plants, and the other with two additional Lhcr subunits attached to the opposite side, indicating an ancient form of PSI-LHCI. Furthermore, the red algal PSI core showed features of both cyanobacterial and higher plant PSI, suggesting an intermediate type during evolution from prokaryotes to eukaryotes. The structure of PsaO, existing in eukaryotic organisms, was identified in the PSI core and binds three chlorophylls and may be important in harvesting energy and in mediating energy transfer from LHCII to the PSI core under state-2 conditions. Individual attaching sites of LHCRs with the core subunits were identified, and each Lhcr was found to contain 11 to 13 chlorophylls and 5 zeaxanthins, which are apparently different from those of LHCs in plant PSI-LHCI. Together, our results reveal unique energy transfer pathways different from those of higher plant PSI-LHCI, its adaptation to the changing environment, and the possible changes of PSI-LHCI during evolution from prokaryotes to eukaryotes.
External links	Proc Natl Acad Sci U S A / PubMed:29632169 / PubMed Central
Methods	EM (single particle)
Resolution	3.63 - 3.82 Å
Structure data	6929 5zgb EMDB-6929, PDB-5zgb: Cryo-EM structure of the red algal PSI-LHCR Method: EM (single particle) / Resolution: 3.63 Å 6930 5zgh EMDB-6930, PDB-5zgh: Cryo-EM structure of the red algal PSI-LHCR Method: EM (single particle) / Resolution: 3.63 Å
Chemicals	ChemComp-CL0: CHLOROPHYLL A ISOMER ChemComp-CLA: CHLOROPHYLL A ChemComp-PQN: PHYLLOQUINONE ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipidYM ChemComp-BCR: BETA-CAROTENE ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-BGC: beta-D-glucopyranose ChemComp-DGD: DIGALACTOSYL DIACYL GLYCEROL (DGDG) ChemComp-3XQ: (2S)-2,3-dihydroxypropyl octadecanoate ChemComp-ZEX: (1R,2S)-4-{(1E,3E,5E,7E,9E,11E,13E,15E,17E)-18-[(4S)-4-hydroxy-2,6,6-trimethylcyclohex-1-en-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaen-1-yl}-2,5,5-trimethylcyclohex-3-en-1-ol ChemComp-1DO*: 1-DODECANOL
Source	Cyanidioschyzon merolae (eukaryote) Red alga (eukaryote) cyanidioschyzon merolae (strain 10d) (eukaryote) cyanidioschyzon merolae strain 10d (eukaryote)
Keywords	PHOTOSYNTHESIS / super-complex / red alga / PSI-5Lhcr