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Structure paper

TitleComplete reconstitution of the diverse pathways of gentamicin B biosynthesis.
Journal, issue, pagesNat. Chem. Biol., Vol. 15, Page 295-303, Year 2019
Publish dateJan 30, 2018 (structure data deposition date)
AuthorsBan, Y.H. / Song, M.C. / Hwang, J.Y. / Shin, H.L. / Kim, H.J. / Hong, S.K. / Lee, N.J. / Park, J.W. / Cha, S.S. / Liu, H.W. / Yoon, Y.J.
External linksNat. Chem. Biol. / PubMed:30643280
MethodsX-ray diffraction
Resolution1.398 - 1.759 Å
Structure data

PDB-5z83:
Crystal structure of GenB1 from Micromonospora echinospora in complex with PLP (internal aldimine)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5z8a:
Crystal structure of GenB1 from Micromonospora echinospora in complex with JI-20A and PLP (external aldimine)
Method: X-RAY DIFFRACTION / Resolution: 1.398 Å

PDB-5z8k:
Crystal structure of an aminotransferase in complex with product-1
Method: X-RAY DIFFRACTION / Resolution: 1.759 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-JIA:
(2~{R},3~{R},4~{R},5~{R})-2-[(1~{S},2~{S},3~{R},4~{S},6~{R})-3-[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(aminomethyl)-3-azany

ChemComp-XXX:
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside / antibiotic*YM

Source
  • micromonospora echinospora (bacteria)
KeywordsTRANSFERASE / aminotransferase / PLP-binding

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