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Structure paper

TitleUnnatural Cytosine Bases Recognized as Thymines by DNA Polymerases by the Formation of the Watson-Crick Geometry.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 58, Page 130-133, Year 2019
Publish dateNov 17, 2017 (structure data deposition date)
AuthorsZeng, H. / Mondal, M. / Song, R. / Zhang, J. / Xia, B. / Liu, M. / Zhu, C. / He, B. / Gao, Y.Q. / Yi, C.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:30407705
MethodsX-ray diffraction
Resolution1.91 - 2.53 Å
Structure data

PDB-5ytc:
Large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with the unnatural base M-fC pair with dATP in the active site
Method: X-RAY DIFFRACTION / Resolution: 2.28 Å

PDB-5ytd:
large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with the natural base pair 5fC:dGTP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5yte:
Large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with with natural dT:dATP base pair
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

PDB-5ytf:
Structure of large fragment of DNA Polymerase I from Thermus aquaticus Host-Guest complex with the unnatural base M-fC pair with dA
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

PDB-5ytg:
Structure of large fragment of DNA Polymerase I from Thermus aquaticus Host-Guest complex with the unnatural base I-fC pair with dA
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-5yth:
Structure of large fragment of DNA Polymerase I from Thermus aquaticus Host-Guest complex with the unnatural base M-fC pair with dG
Method: X-RAY DIFFRACTION / Resolution: 2.53 Å

PDB-5z3n:
Structure of large fragment of DNA Polymerase I from Thermus aquaticus Host-Guest complex with the unnatural base 5fC pair with dA
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

Chemicals

ChemComp-DTP:
2'-DEOXYADENOSINE 5'-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

ChemComp-GOL:
GLYCEROL

Source
  • thermus aquaticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / DNA / REPLICATION / TRANSFERASE-DNA complex / REPLICATION/DNA / REPLICATION-DNA complex / DNA polymerase / Host-Guest complex

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