| タイトル | Structural and mechanistic insights into homocysteine degradation by a mutant of methionine gamma-lyase based on substrate-assisted catalysis |
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| ジャーナル・号・ページ | Protein Sci., Vol. 26, Page 1224-1230, Year 2017 |
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| 掲載日 | 2017年2月2日 (構造データの登録日) |
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著者 | Sato, D. / Shiba, T. / Yunoto, S. / Furutani, K. / Fukumoto, M. / Kudou, D. / Tamura, T. / Inagaki, K. / Harada, S. |
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リンク | Protein Sci. / PubMed:28329912 |
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| 手法 | X線回折 |
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| 解像度 | 1.7 - 2.7 Å |
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| 構造データ | PDB-5x2v: Crystal structure of Pseudomonas putida methionine gamma-lyase wild type without sulfate ion 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-5x2w: Crystal structure of Pseudomonas putida methionine gamma-lyase wild type with L-methionine intermediates 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-5x2x: Crystal structure of Pseudomonas putida methionine gamma-lyase wild type with L-homocysteine intermediates 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-5x2y: Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant without sulfate ion 手法: X-RAY DIFFRACTION / 解像度: 1.79 Å PDB-5x2z: Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-methionine intermediates 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-5x30: Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-homocysteine intermediates. 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å |
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| 化合物 | ChemComp-3LM: (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid
ChemComp-4LM: (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid
ChemComp-7XF: (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid
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| 由来 | pseudomonas putida (バクテリア)
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キーワード | LYASE / Pyridoxal 5'-phosphate |
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