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Structure paper

TitleCo-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 9573-9582, Year 2017
Publish dateJul 20, 2017 (structure data deposition date)
AuthorsAlmutairi, M.M. / Svetlov, M.S. / Hansen, D.A. / Khabibullina, N.F. / Klepacki, D. / Kang, H.Y. / Sherman, D.H. / Vazquez-Laslop, N. / Polikanov, Y.S. / Mankin, A.S.
External linksNucleic Acids Res. / PubMed:28934499
MethodsX-ray diffraction
Resolution2.6 - 2.703 Å
Structure data

PDB-5wis:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.703 Å

PDB-5wit:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-MT9:
(3R,4S,5S,7R,9E,11S,12R)-12-ethyl-11-hydroxy-3,5,7,11-tetramethyl-2,8-dioxooxacyclododec-9-en-4-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside

ChemComp-K:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-AQJ:
(3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside

Source
  • escherichia coli (E. coli)
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus (bacteria)
  • enterobacteria phage t4 (virus)
KeywordsRIBOSOME / macrolide / antibiotic / inhibitor / inhibition of translation / peptidyl transferase center

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