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Structure paper

TitleRational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase.
Journal, issue, pagesSci Rep, Vol. 7, Page 16745-16745, Year 2017
Publish dateMay 10, 2017 (structure data deposition date)
AuthorsJacquet, P. / Hiblot, J. / Daude, D. / Bergonzi, C. / Gotthard, G. / Armstrong, N. / Chabriere, E. / Elias, M.
External linksSci Rep / PubMed:29196634
MethodsX-ray diffraction
Resolution1.4 - 2.95 Å
Structure data

PDB-5vri:
Crystal structure of SsoPox AsA6 mutant (F46L-C258A-W263M-I280T) - closed form
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-5vrk:
Crystal structure of SsoPox AsA6 mutant (F46L-C258A-W263M-I280T) - open form
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5vsa:
Crystal structure of SsoPox AsA1 mutant (C258L-I261F-W263A)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5w3u:
Crystal structure of SsoPox AsB5 mutant (V27A-I76T-Y97W-Y99F-L130P-L226V)
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5w3w:
Crystal structure of SsoPox AsD6 mutant (V27A-Y97W-L228M-W263M) - open form
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-5w3z:
Crystal structure of SsoPox AsC6 mutant (L72I-Y99F-I122L-L228M-F229S-W263L)
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

Chemicals

ChemComp-CO:
Unknown entry

ChemComp-FE2:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • sulfolobus solfataricus (archaea)
KeywordsHYDROLASE / lactonase / phosphotriesterase / mutants / quorum sensing / organophosphate / organophosphorous / insecticides.

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