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TitleCautionary Tale of Using Tris(alkyl)phosphine Reducing Agents with NAD+-Dependent Enzymes.
Journal, issue, pagesBiochemistry, Year 2020
Publish dateMay 5, 2017 (structure data deposition date)
AuthorsPatel, S.M. / Smith, T.G. / Morton, M. / Stiers, K.M. / Seravalli, J. / Mayclin, S.J. / Edwards, T.E. / Tanner, J.J. / Becker, D.F.
External linksBiochemistry / PubMed:32841567
MethodsX-ray diffraction
Resolution1.45 Å
Structure data

PDB-5vps:
Crystal structure of an SDR from Burkholderia ambifaria in complex with NADPH with a TCEP adduct
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

Chemicals

ChemComp-9G1:
[(4~{S})-3-aminocarbonyl-1-[(2~{R},3~{R},4~{S},5~{R})-5-[[[[(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]piperidin-4-yl]-tris(3-hydroxy-3-oxopropyl)phosphanium

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • burkholderia ambifaria (strain mc40-6) (bacteria)
KeywordsOXIDOREDUCTASE / SGCID / short chain / dehydrogenase / reductase / NADP / NADPH / TCEP / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID

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