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Structure paper

TitleStructural basis for the D-stereoselectivity of human DNA polymerase beta.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 6228-6237, Year 2017
Publish dateNov 30, 2016 (structure data deposition date)
AuthorsVyas, R. / Reed, A.J. / Raper, A.T. / Zahurancik, W.J. / Wallenmeyer, P.C. / Suo, Z.
External linksNucleic Acids Res. / PubMed:28402499
MethodsX-ray diffraction
Resolution1.79 - 2.3 Å
Structure data

PDB-5u2r:
PRECATALYTIC TERNARY COMPLEX OF HUMAN DNA POLYMERASE BETA WITH GAPPED DNA SUBSTARTE, INCOMING L-DCTP AND CA2+
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5u2s:
Pre-catalytic ternary complex of Human DNA Polymerase Beta With Gapped DNA substrate incoming (-)3TC-TP and Ca2+.
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5u2t:
Pre-catalytic ternary complex of Human DNA Polymerase Beta With Gapped DNA substrate incoming (-)FTC-TP and Ca2+.
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

Chemicals

ChemComp-1S0:
4-amino-1-{2-deoxy-5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-L-erythro-pentofuranosyl}pyrimidin-2(1H)-one

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-1RZ:
Lamivudine Triphosphate

ChemComp-1RY:
[[(2R,5S)-5-(4-azanyl-5-fluoranyl-2-oxidanylidene-pyrimidin-1-yl)-1,3-oxathiolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / X-FAMILY / POL BETA / DNA POLYMERASE BETA / TRANSFERASE-DNA COMPLEX

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