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Structure paper

TitleStructures of two distinct conformations of holo-non-ribosomal peptide synthetases.
Journal, issue, pagesNature, Vol. 529, Page 235-238, Year 2016
Publish dateMay 20, 2015 (structure data deposition date)
AuthorsDrake, E.J. / Miller, B.R. / Shi, C. / Tarrasch, J.T. / Sundlov, J.A. / Allen, C.L. / Skiniotis, G. / Aldrich, C.C. / Gulick, A.M.
External linksNature / PubMed:26762461
MethodsX-ray diffraction
Resolution2.7 - 2.9 Å
Structure data

PDB-4zxh:
Crystal Structure of holo-AB3403 a four domain nonribosomal peptide synthetase from Acinetobacter Baumanii
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-4zxi:
Crystal Structure of holo-AB3403 a four domain nonribosomal peptide synthetase bound to AMP and Glycine
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-5t3d:
Crystal structure of holo-EntF a nonribosomal peptide synthetase in the thioester-forming conformation
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE

ChemComp-PX4:
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DMPC, phospholipid*YM

ChemComp-K:
Unknown entry

ChemComp-NI:
NICKEL (II) ION

ChemComp-HOH:
WATER

ChemComp-GLY:
GLYCINE

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-XP4:
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE / DMPA, phospholipid*YM

ChemComp-MG:
Unknown entry

ChemComp-75C:
5'-({[(2R,3S)-3-amino-4-hydroxy-2-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}butyl]sulfonyl}amino)-5'-deoxyadenosine

Source
  • acinetobacter baumannii (strain ab307-0294) (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / Nonribosomal peptide synthetase / NRPS / Condensation / Adenylation / PCP / Thioesterase / phosphopantetheine

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