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Structure paper

TitleStructural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
Journal, issue, pagesChem. Commun. (Camb. ), Vol. 53, Page 12704-12707, Year 2017
Publish dateJun 9, 2017 (structure data deposition date)
AuthorsBetz, K. / Nilforoushan, A. / Wyss, L.A. / Diederichs, K. / Sturla, S.J. / Marx, A.
External linksChem. Commun. (Camb. ) / PubMed:29136072
MethodsX-ray diffraction
Resolution1.8 - 2 Å
Structure data

PDB-5o7t:
Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5oxj:
Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a O6-MeG:BenziTP base pair
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-DCP:
2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

ChemComp-NZI:
[(2~{R},3~{S},5~{R})-3-oxidanyl-5-(2-oxidanylidene-3~{H}-benzimidazol-1-yl)oxolan-2-yl]methyl [oxidanyl(phosphonooxy)phosphoryl] hydrogen phosphate

ChemComp-ACT:
ACETATE ION

Source
  • thermus aquaticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE / DNA polymerase

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