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Structure paper

TitleAn allosteric binding site of the alpha 7 nicotinic acetylcholine receptor revealed in a humanized acetylcholine-binding protein.
Journal, issue, pagesJ. Biol. Chem., Vol. 293, Page 2534-2545, Year 2018
Publish dateAug 23, 2017 (structure data deposition date)
AuthorsDelbart, F. / Brams, M. / Gruss, F. / Noppen, S. / Peigneur, S. / Boland, S. / Chaltin, P. / Brandao-Neto, J. / von Delft, F. / Touw, W.G. ...Delbart, F. / Brams, M. / Gruss, F. / Noppen, S. / Peigneur, S. / Boland, S. / Chaltin, P. / Brandao-Neto, J. / von Delft, F. / Touw, W.G. / Joosten, R.P. / Liekens, S. / Tytgat, J. / Ulens, C.
External linksJ. Biol. Chem. / PubMed:29237730
MethodsX-ray diffraction
Resolution2.5 - 3.1 Å
Structure data

PDB-5oug:
Humanized alpha-AChBP (acetylcholine binding protein) in complex with lobeline and allosteric binder fragment 4.
Method: X-RAY DIFFRACTION / Resolution: 2.57 Å

PDB-5ouh:
Humanized alpha-AChBP (acetylcholine binding protein) in complex with lobeline.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5oui:
Humanized alpha-AChBP (acetylcholine binding protein) in complex with allosteric binder fragment CU2017
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-L0B:
Alpha-Lobeline / alkaloid*YM

ChemComp-9Z0:
4,5-dibromo-N-(3-hydroxypropyl)-1H-pyrrole-2-carboxamide

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-HOH:
WATER

ChemComp-AVB:
(3~{R})-~{N}-(5-bromanylpyridin-2-yl)piperidine-3-carboxamide

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • homo sapiens (human)
  • lymnaea stagnalis (great pond snail)
KeywordsCHOLINE-BINDING PROTEIN / acetylcholine binding protein / nicotinic acetylcholine receptor

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