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Structure paper

TitleStructural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page E6526-E6535, Year 2017
Publish dateJul 26, 2013 (structure data deposition date)
AuthorsPluta, R. / Boer, D.R. / Lorenzo-Diaz, F. / Russi, S. / Gomez, H. / Fernandez-Lopez, C. / Perez-Luque, R. / Orozco, M. / Espinosa, M. / Coll, M.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28739894
MethodsX-ray diffraction
Resolution1.9 - 3.1 Å
Structure data

PDB-4lvi:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4lvj:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 5.5
Method: X-RAY DIFFRACTION / Resolution: 2.17 Å

PDB-4lvk:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt+3'Phosphate). Mn-bound crystal structure at pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 2.37 Å

PDB-4lvl:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt+3'Thiophosphate). Mn-bound crystal structure at pH 6.8
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4lvm:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (23nt). Mn-bound crystal structure at pH 6.5
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-5n2q:
MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • streptococcus agalactiae (bacteria)
  • synthetic dna (others)
  • plasmid pmv158 (others)
KeywordsDNA BINDING PROTEIN/DNA / Protein-DNA complex / Pfam Family: Mob_Pre (PF01076). MOBv Family of Relaxases / relaxase/endonuclease / oriT DNA / DNA BINDING PROTEIN-DNA complex / Pfam Family Mob_Pre (PF01076) / MOBv Family / MOB Relaxase Family / MOBv / Pfam Family: Mob_Pre (PF01076). MOB Relaxase Family: MOBv / DNA BINDING PROTEIN / relaxase / nuclease / conjugation

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