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Structure paper

TitleImproved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.
Journal, issue, pagesActa Crystallogr F Struct Biol Commun, Vol. 74, Page 307-314, Year 2018
Publish dateFeb 4, 2017 (structure data deposition date)
AuthorsPichlo, C. / Toelzer, C. / Chojnacki, K. / Ocal, S. / Uthoff, M. / Ruegenberg, S. / Hermanns, T. / Schacherl, M. / Denzel, M.S. / Hofmann, K. ...Pichlo, C. / Toelzer, C. / Chojnacki, K. / Ocal, S. / Uthoff, M. / Ruegenberg, S. / Hermanns, T. / Schacherl, M. / Denzel, M.S. / Hofmann, K. / Niefind, K. / Baumann, U.
External linksActa Crystallogr F Struct Biol Commun / PubMed:29717999
MethodsX-ray diffraction
Resolution1.2 - 1.39 Å
Structure data

PDB-5n12:
Crystal structure of TCE treated rPPEP-1
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

PDB-6fsj:
Crystal structure of TCE-treated Lysozyme
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-6fsm:
Crystal structure of TCE-treated Thermolysin
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-8FH:
2,2,2-tris-chloroethanol

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CIT:
CITRIC ACID

ChemComp-CA:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-VAL:
VALINE

ChemComp-LYS:
LYSINE

Source
  • peptoclostridium difficile 630 (bacteria)
  • gallus gallus (chicken)
  • geobacillus stearothermophilus (bacteria)
KeywordsHYDROLASE / Metalloprotease / TCE / Zinc / Clostridium difficle / TCE-treatment / Lysozyme

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