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Structure paper

TitleStructural insights into the functional cycle of the ATPase module of the 26S proteasome.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 114, Issue 6, Page 1305-1310, Year 2017
Publish dateFeb 7, 2017
AuthorsMarc Wehmer / Till Rudack / Florian Beck / Antje Aufderheide / Günter Pfeifer / Jürgen M Plitzko / Friedrich Förster / Klaus Schulten / Wolfgang Baumeister / Eri Sakata /
PubMed AbstractIn eukaryotic cells, the ubiquitin-proteasome system (UPS) is responsible for the regulated degradation of intracellular proteins. The 26S holocomplex comprises the core particle (CP), where ...In eukaryotic cells, the ubiquitin-proteasome system (UPS) is responsible for the regulated degradation of intracellular proteins. The 26S holocomplex comprises the core particle (CP), where proteolysis takes place, and one or two regulatory particles (RPs). The base of the RP is formed by a heterohexameric AAA ATPase module, which unfolds and translocates substrates into the CP. Applying single-particle cryo-electron microscopy (cryo-EM) and image classification to samples in the presence of different nucleotides and nucleotide analogs, we were able to observe four distinct conformational states (s1 to s4). The resolution of the four conformers allowed for the construction of atomic models of the AAA ATPase module as it progresses through the functional cycle. In a hitherto unobserved state (s4), the gate controlling access to the CP is open. The structures described in this study allow us to put forward a model for the 26S functional cycle driven by ATP hydrolysis.
External linksProc Natl Acad Sci U S A / PubMed:28115689 / PubMed Central
MethodsEM (single particle)
Resolution4.1 - 7.8 Å
Structure data

3534

5mp9

5mpd

EMDB-3534, PDB-5mp9, PDB-5mpd:
26S proteasome in presence of ATP (s1)
Method: EM (single particle) / Resolution: 4.1 Å

3535

5mpa

5mpe

EMDB-3535, PDB-5mpa, PDB-5mpe:
26S proteasome in presence of ATP (s2)
Method: EM (single particle) / Resolution: 4.5 Å

3536

5mpb

EMDB-3536, PDB-5mpb:
26S proteasome in presence of AMP-PNP (s3)
Method: EM (single particle) / Resolution: 7.8 Å

3537

5mpc

EMDB-3537, PDB-5mpc:
26S proteasome in presence of BeFx (s4)
Method: EM (single particle) / Resolution: 7.7 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • Saccharomyces cerevisiae S288c (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsHYDROLASE / Macromolecular complex / 26S proteasome / Protease

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