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Structure paper

TitleNonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 7355-7360, Year 2017
Publish dateDec 5, 2016 (structure data deposition date)
AuthorsArragain, S. / Bimai, O. / Legrand, P. / Caillat, S. / Ravanat, J.L. / Touati, N. / Binet, L. / Atta, M. / Fontecave, M. / Golinelli-Pimpaneau, B.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28655838
MethodsX-ray diffraction
Resolution2.5 - 2.79 Å
Structure data

PDB-5mko:
[2Fe-2S] cluster containing TtuA in complex with AMP.
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-5mkp:
Non redox thiolation in transfer RNAs occuring via sulfur activation by a [4Fe-4S] cluster
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5mkq:
TtuA enzyme containing a [4Fe-4S]
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

Chemicals

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-ZN:
Unknown entry

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-HOH:
WATER

ChemComp-Q46:
Fe4 H S5

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • pyrococcus horikoshii (strain atcc 700860 / dsm 12428 / jcm 9974 / nbrc 100139 / ot-3) (archaea)
  • pyrococcus horikoshii ot3 (archaea)
KeywordsRNA / TtuA / PH0300 / AMP / 2Fe-2S / Thiolation / tRNA modification enzyme / [Fe-S] cluster / iron-sulfur cluster / [4Fe-5S] / sulfur insertion / tRNA modification / thiolation reaction / OXIDOREDUCTASE / Apo / 4Fe-4S / Fe-edge / anomal / tRNA thiolation

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