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TitleMolecular Structures of Transcribing RNA Polymerase I.
Journal, issue, pagesMol Cell, Vol. 64, Issue 6, Page 1135-1143, Year 2016
Publish dateDec 15, 2016
AuthorsLucas Tafur / Yashar Sadian / Niklas A Hoffmann / Arjen J Jakobi / Rene Wetzel / Wim J H Hagen / Carsten Sachse / Christoph W Müller /
PubMed AbstractRNA polymerase I (Pol I) is a 14-subunit enzyme that solely synthesizes pre-ribosomal RNA. Recently, the crystal structure of apo Pol I gave unprecedented insight into its molecular architecture. ...RNA polymerase I (Pol I) is a 14-subunit enzyme that solely synthesizes pre-ribosomal RNA. Recently, the crystal structure of apo Pol I gave unprecedented insight into its molecular architecture. Here, we present three cryo-EM structures of elongating Pol I, two at 4.0 Å and one at 4.6 Å resolution, and a Pol I open complex at 3.8 Å resolution. Two modules in Pol I mediate the narrowing of the DNA-binding cleft by closing the clamp domain. The DNA is bound by the clamp head and by the protrusion domain, allowing visualization of the upstream and downstream DNA duplexes in one of the elongation complexes. During formation of the Pol I elongation complex, the bridge helix progressively folds, while the A12.2 C-terminal domain is displaced from the active site. Our results reveal the conformational changes associated with elongation complex formation and provide additional insight into the Pol I transcription cycle.
External linksMol Cell / PubMed:27867008 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 4.6 Å
Structure data

3446

5m5w

EMDB-3446, PDB-5m5w:
RNA Polymerase I open complex
Method: EM (single particle) / Resolution: 3.8 Å

3447

5m5x

EMDB-3447, PDB-5m5x:
RNA Polymerase I elongation complex 1
Method: EM (single particle) / Resolution: 4.0 Å

3448

5m5y

EMDB-3448, PDB-5m5y:
RNA Polymerase I elongation complex 2
Method: EM (single particle) / Resolution: 4.0 Å

3449

5m64

EMDB-3449, PDB-5m64:
RNA Polymerase I elongation complex with A49 tandem winged helix domain
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsTRANSCRIPTION / RNA Polymerase / RNA Polymerase I / elongation

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