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Structure paper

TitleThe RNA lariat debranching enzyme Dbr1: metal dependence and branched RNA co-crystal structures
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Year 2016
Publish dateMay 25, 2016 (structure data deposition date)
AuthorsClark, N.E. / Katolik, A. / Roberts, K. / Taylor, A.B. / Holloway, S.P. / Schuermann, J.P. / Montemayor, E.J. / Stevens, S.W. / Fitzpatrick, P.F. / Damha, M.J. / Hart, P.J.
External linksProc. Natl. Acad. Sci. USA / Search PubMed
MethodsX-ray diffraction
Resolution2.08 - 2.64 Å
Structure data

PDB-5k71:
apo Dbr1
Method: X-RAY DIFFRACTION / Resolution: 2.57 Å

PDB-5k73:
as-isolated Dbr1 with Fe(II) and Zn(II)
Method: X-RAY DIFFRACTION / Resolution: 2.08 Å

PDB-5k78:
Dbr1 in complex with 16-mer branched RNA
Method: X-RAY DIFFRACTION / Resolution: 2.64 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-FE2:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-OH:
HYDROXIDE ION

Source
  • entamoeba histolytica (eukaryote)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsHYDROLASE / metalloenzyme / apo-enzyme / HYDROLASE/RNA / complex / branched RNA / HYDROLASE-RNA complex

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