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Structure paper

TitleNeutron structures of the Helicobacter pylori 5'-methylthioadenosine nucleosidase highlight proton sharing and protonation states.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 113, Page 13756-13761, Year 2016
Publish dateJul 1, 2015 (structure data deposition date)
AuthorsBanco, M.T. / Mishra, V. / Ostermann, A. / Schrader, T.E. / Evans, G.B. / Kovalevsky, A. / Ronning, D.R.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:27856757
MethodsX-ray diffraction / neutron diffraction
Resolution1.399 - 2.6 Å
Structure data

PDB-5ccd:
Joint X-ray/neutron structure of MTAN D198N complex with SAH
Method: X-RAY DIFFRACTION / NEUTRON DIFFRACTION / Resolution: 2.2 Å

PDB-5cce:
Joint X-ray/neutron structure of wild type MTAN complexed with SRH and adenine
Method: NEUTRON DIFFRACTION / X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5jpc:
Joint X-ray/neutron structure of MTAN complex with Formycin A
Method: NEUTRON DIFFRACTION / X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5k1z:
Joint X-ray/neutron structure of MTAN complex with p-ClPh-Thio-DADMe-ImmA
Method: NEUTRON DIFFRACTION / X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5kb3:
1.4 A resolution structure of Helicobacter Pylori MTAN in complexed with p-ClPh-DADMe-ImmA
Method: X-RAY DIFFRACTION / Resolution: 1.399 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-DOD:
DEUTERATED WATER

ChemComp-2WP:
S-ribosylhomocysteine

ChemComp-ADE:
ADENINE

ChemComp-D8U:
Unknown entry

ChemComp-FMC:
(1S)-1-(7-amino-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro-D-ribitol

ChemComp-4CT:
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(4-chlorophenyl)sulfanyl]methyl}pyrrolidin-3-ol

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • helicobacter pylori (strain j99 / atcc 700824) (bacteria)
  • helicobacter pylori (bacteria)
KeywordsHYDROLASE / Helicobacter pylori / Neutron / S-Adenosylhomocysteine / N-Glycosyl Hydrolases / binding sites / n-glycosyl neutron / deuterium / nucleosidase / Joint Neutron and X-ray / neutron crystallography

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