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TitleMulti-Parameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity and Activity of an Epoxide Hydrolase
Journal, issue, pagesTo be published
Publish dateMay 4, 2016 (structure data deposition date)
AuthorsLi, G. / Zhang, H. / Sun, Z. / Liu, X. / Reetz, M.T.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.5 - 2.5 Å
Structure data

PDB-5jpp:
Structure of limonene epoxide hydrolase mutant - H-2-H5
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5jpu:
Structure of limonene epoxide hydrolase mutant - H-2-H5 complex with (S,S)-cyclohexane-1,2-diol
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-3ZQ:
(1S,2S)-cyclohexane-1,2-diol

Source
  • rhodococcus erythropolis (bacteria)
KeywordsHYDROLASE / Epoxide Hydrolase / Enantioselectivity / Complex

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