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Structure paper

TitleRegulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.
Journal, issue, pagesElife, Vol. 5, Year 2016
Publish dateMar 18, 2016 (structure data deposition date)
AuthorsTrajtenberg, F. / Imelio, J.A. / Machado, M.R. / Larrieux, N. / Marti, M.A. / Obal, G. / Mechaly, A.E. / Buschiazzo, A.
External linksElife / PubMed:27938660
MethodsX-ray diffraction
Resolution2.9 - 3.2 Å
Structure data

PDB-5iuj:
Crystal structure of the DesK-DesR complex in the phosphotransfer state with low Mg2+ (20 mM)
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-5iuk:
Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM)
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-5iul:
Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM) and BeF3
Method: X-RAY DIFFRACTION / Resolution: 3.153 Å

PDB-5ium:
Crystal structure of phosphorylated DesKC
Method: X-RAY DIFFRACTION / Resolution: 3.162 Å

Chemicals

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • bacillus subtilis (bacteria)
  • bacillus subtilis (strain 168) (bacteria)
KeywordsTRANSFERASE / Two-component regulatory system / Kinase / Response regulator / Phosphotransfer complex / Phosphotransfer / TRANSFERASE/GENE REGULATION / TRANSFERASE-GENE REGULATION complex / four-helix bundle / GHL ATPase domain / Cell membrane / Membrane / Phosphoprotein / Transmembrane

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