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Structure paper

TitleStructure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO.
Journal, issue, pagesPlos Biol., Vol. 14, Page e1002464-e1002464, Year 2016
Publish dateNov 11, 2015 (structure data deposition date)
AuthorsBoyaci, H. / Shah, T. / Hurley, A. / Kokona, B. / Li, Z. / Ventocilla, C. / Jeffrey, P.D. / Semmelhack, M.F. / Fairman, R. / Bassler, B.L. / Hughson, F.M.
External linksPlos Biol. / PubMed:27219477
MethodsX-ray diffraction
Resolution1.421 - 1.8 Å
Structure data

PDB-5ep0:
Quorum-Sensing Signal Integrator LuxO - Receiver+Catalytic Domains
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5ep1:
Quorum-Sensing Signal Integrator LuxO - Catalytic Domain
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5ep2:
Quorum-Sensing Signal Integrator LuxO - Catalytic Domain in Complex with AzaU Inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.421 Å

PDB-5ep3:
Quorum-Sensing Signal Integrator LuxO - Catalytic Domain Bound to CV-133 Inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5ep4:
Structure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

ChemComp-AZU:
2,2-dimethylpropyl 2-[[3,5-bis(oxidanylidene)-2~{H}-1,2,4-triazin-6-yl]sulfanyl]ethanoate

ChemComp-5QT:
2,2-dimethylpropyl 2-[(3-oxidanylidene-5-sulfanylidene-2~{H}-1,2,4-triazin-6-yl)amino]ethanoate

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • photobacterium angustum (bacteria)
KeywordsTRANSCRIPTION / Quorum sensing / AAA+ protein / receiver domain / ATPase / catalytic domain / TRANSCRIPTION/TRANSCRIPTION inhibitor / inhibitor / TRANSCRIPTION-TRANSCRIPTION inhibitor complex

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