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TitleArtificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases.
Journal, issue, pagesProtein Sci., Vol. 25, Page 1241-1248, Year 2016
Publish dateOct 7, 2015 (structure data deposition date)
AuthorsCahn, J.K. / Brinkmann-Chen, S. / Buller, A.R. / Arnold, F.H.
External linksProtein Sci. / PubMed:26644020
MethodsX-ray diffraction
Resolution1.94 Å
Structure data

PDB-5e4r:
Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-40E:
oxo(propan-2-ylamino)acetic acid

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • ignisphaera aggregans (strain dsm 17230 / jcm 13409 / aq1.s1) (archaea)
KeywordsOXIDOREDUCTASE / ketol-acid reductiosomerase / protein knot / psuedodimer / protein engineering / chimera protein

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