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Structure paper

TitleRational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.
Journal, issue, pagesSci Rep, Vol. 6, Page 18459-18459, Year 2016
Publish dateSep 18, 2015 (structure data deposition date)
AuthorsEl Khatib, M. / Martins, A. / Bourgeois, D. / Colletier, J.P. / Adam, V.
External linksSci Rep / PubMed:26732634
MethodsX-ray diffraction
Resolution1.45 - 2.35 Å
Structure data

PDB-5dtx:
Crystal structure of rsEGFP2 in the fluorescent on-state
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-5dty:
Crystal structure of rsEGFP2 in the non-fluorescent off-state
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5dtz:
Crystal structure of rsFolder in the fluorescent on-state
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5du0:
Crystal structure of rsFolder in the non-fluorescent off-state
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / GFP / reversibly switchable / cis chromophore / trans chromophore

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