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TitleBlue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence.
Journal, issue, pagesElife, Vol. 5, Page e11860, Year 2016
Publish dateJan 12, 2016
AuthorsUdo Heintz / Ilme Schlichting /
PubMed AbstractThe design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. ...The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To understand allosteric communication between sensor and effector domains, characterization of all relevant signaling states is required. Here, we describe the mechanism of light-dependent DNA binding of the light-oxygen-voltage (LOV) transcription factor Aureochrome 1a from Phaeodactylum tricornutum (PtAu1a) and present crystal structures of a dark state LOV monomer and a fully light-adapted LOV dimer. In combination with hydrogen/deuterium-exchange, solution scattering data and DNA-binding experiments, our studies reveal a light-sensitive interaction between the LOV and basic region leucine zipper DNA-binding domain that together with LOV dimerization results in modulation of the DNA affinity of PtAu1a. We discuss the implications of these results for the design of synthetic LOV-based photosensors with application in optogenetics.
External linksElife / PubMed:26754770 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2.5 - 2.7 Å
Structure data

SASDB44:
Aureochrome 1a from P. tricornutum, amino acids 148-378 (N-terminal truncation), dark state
Method: SAXS/SANS

PDB-5dkk:
Structure of the dark-state monomer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5dkl:
Structure of the light-state dimer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-HOH:
WATER / Water

Source
  • phaeodactylum tricornutum (Diatom)
KeywordsSIGNALING PROTEIN / LOV DNA binding photoreceptor / flavoprotein / transcription / light oxygen voltage DNA binding photoreceptor blue light

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