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Structure paper

TitleThe cubicon method for concentrating membrane proteins in the cubic mesophase.
Journal, issue, pagesNat Protoc, Vol. 12, Page 1745-1762, Year 2017
Publish dateAug 12, 2015 (structure data deposition date)
AuthorsMa, P. / Weichert, D. / Aleksandrov, L.A. / Jensen, T.J. / Riordan, J.R. / Liu, X. / Kobilka, B.K. / Caffrey, M.
External linksNat Protoc / PubMed:28771236
MethodsX-ray diffraction
Resolution2.4 - 3.2 Å
Structure data

PDB-5d6i:
DgkA - CIM
Method: X-RAY DIFFRACTION / Resolution: 3.091 Å

PDB-5d6k:
PepT - CIM
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5d6l:
beta2AR-T4L - CIM
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-5iyu:
AlgE_CIM
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-97M:
(2R)-2,3-dihydroxypropyl (9Z)-hexadec-9-enoate

ChemComp-97N:
(2S)-2,3-dihydroxypropyl (9Z)-hexadec-9-enoate

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-CAU:
(2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol

ChemComp-BU1:
1,4-BUTANEDIOL

ChemComp-ACM:
ACETAMIDE

ChemComp-CLR:
CHOLESTEROL

ChemComp-PLM:
PALMITIC ACID

ChemComp-12P:
DODECAETHYLENE GLYCOL / precipitant*YM

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-PE5:
3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL / precipitant*YM

ChemComp-NA:
Unknown entry

Source
  • escherichia coli (E. coli)
  • streptococcus thermophilus (strain atcc baa-250 / lmg 18311) (bacteria)
  • homo sapiens (human)
  • enterobacteria phage t4 (virus)
  • pseudomonas aeruginosa pao1 (bacteria)
KeywordsTRANSFERASE / DIACYLGLYCEROL KINASE / CLLD / LIPID METABOLISM / TRANSPORT PROTEIN / ALPHA-HELICAL / Major Facilitator Superfamily (MFS) Transporters / PepTSt Oligopeptide-proton symporter (POT family) / MEMBRANE PROTEIN/HYDROLASE / G Protein-Coupled Receptors (GPCRs) / Beta-2 adrenergic receptor / membrane protein-hydrolase complex / MEMBRANE PROTEIN / alginate transporter

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