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Structure paper

TitleCrystal Structures of Apo and Liganded 4-Oxalocrotonate Decarboxylase Uncover a Structural Basis for the Metal-Assisted Decarboxylation of a Vinylogous beta-Keto Acid.
Journal, issue, pagesBiochemistry, Vol. 55, Page 2632-2645, Year 2016
Publish dateAug 5, 2015 (structure data deposition date)
AuthorsGuimaraes, S.L. / Coitinho, J.B. / Costa, D.M. / Araujo, S.S. / Whitman, C.P. / Nagem, R.A.
External linksBiochemistry / PubMed:27082660
MethodsX-ray diffraction
Resolution1.571 - 1.936 Å
Structure data

PDB-5d2f:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - apo form
Method: X-RAY DIFFRACTION / Resolution: 1.738 Å

PDB-5d2g:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with magnesium
Method: X-RAY DIFFRACTION / Resolution: 1.897 Å

PDB-5d2h:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with magnesium and alpha-ketoglutarate
Method: X-RAY DIFFRACTION / Resolution: 1.936 Å

PDB-5d2i:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with calcium and acetate
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-5d2j:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with magnesium and adipate
Method: X-RAY DIFFRACTION / Resolution: 1.718 Å

PDB-5d2k:
4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with magnesium and 2-oxoadipate
Method: X-RAY DIFFRACTION / Resolution: 1.571 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-CA:
Unknown entry

ChemComp-0L1:
hexanedioic acid

ChemComp-OOG:
2-OXOADIPIC ACID

Source
  • pseudomonas putida (bacteria)
KeywordsLYASE / naphthalene degradation

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