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Structure paper

TitleStructure of iridoid synthase in complex with NADP(+)/8-oxogeranial reveals the structural basis of its substrate specificity.
Journal, issue, pagesJ. Struct. Biol., Vol. 194, Page 224-230, Year 2016
Publish dateJul 20, 2015 (structure data deposition date)
AuthorsQin, L. / Zhu, Y. / Ding, Z. / Zhang, X. / Ye, S. / Zhang, R.
External linksJ. Struct. Biol. / PubMed:26868105
MethodsX-ray diffraction
Resolution2.2 - 2.65 Å
Structure data

PDB-5coa:
Crystal structure of iridoid synthase at 2.2-angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5cob:
Crystal structure of iridoid synthase in complex with NADP+ and 8-oxogeranial at 2.65-angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

Chemicals

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-XOG:
(2E,6E)-2,6-dimethylocta-2,6-dienedial

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • catharanthus roseus (Madagascar periwinkle)
KeywordsOXIDOREDUCTASE / SDR / P5bR / Rossmann fold / 1-4 addition

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