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Structure paper

TitleUsing mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases.
Journal, issue, pagesNat Commun, Vol. 7, Page 10911-10911, Year 2016
Publish dateJul 16, 2015 (structure data deposition date)
Authorsvan der Meer, J.Y. / Poddar, H. / Baas, B.J. / Miao, Y. / Rahimi, M. / Kunzendorf, A. / van Merkerk, R. / Tepper, P.G. / Geertsema, E.M. / Thunnissen, A.M. ...van der Meer, J.Y. / Poddar, H. / Baas, B.J. / Miao, Y. / Rahimi, M. / Kunzendorf, A. / van Merkerk, R. / Tepper, P.G. / Geertsema, E.M. / Thunnissen, A.M. / Quax, W.J. / Poelarends, G.J.
External linksNat Commun / PubMed:26952338
MethodsX-ray diffraction
Resolution2.3 - 2.71 Å
Structure data

PDB-5cln:
Crystal structure of a 4-oxalocrotonate tautomerase mutant at 2.7 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 2.71 Å

PDB-5clo:
Crystal structure of a 4-oxalocrotonate tautomerase mutant in complex with nitrostyrene at 2.3 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NS8:
trans beta nitrostyrene

Source
  • pseudomonas putida (bacteria)
KeywordsISOMERASE / 4-Oxalocrotonate tautomerase / beta-alpha-beta structural motif / tautomerase superfamily

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