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TitleCrystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic beta-glucosidase Td2F2.
Journal, issue, pagesFebs J., Vol. 283, Page 2340-2353, Year 2016
Publish dateAug 21, 2013 (structure data deposition date)
AuthorsMatsuzawa, T. / Jo, T. / Uchiyama, T. / Manninen, J.A. / Arakawa, T. / Miyazaki, K. / Fushinobu, S. / Yaoi, K.
External linksFebs J. / PubMed:27092463
MethodsX-ray diffraction
Resolution1.1 - 1.9 Å
Structure data

PDB-3wh5:
Crystal structure of GH1 beta-glucosidase Td2F2
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3wh6:
Crystal structure of GH1 beta-glucosidase Td2F2 glucose complex
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3wh7:
Crystal structure of GH1 beta-glucosidase Td2F2 L-fucose complex
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-3wh8:
Crystal structure of GH1 beta-glucosidase Td2F2 isofagomine complex
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5ayb:
Crystal structure of GH1 Beta-Glucosidase TD2F2 N223G mutant
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5ayi:
Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-GLC:
alpha-D-glucopyranose

ChemComp-FCB:
beta-D-fucopyranose

ChemComp-IFM:
5-HYDROXYMETHYL-3,4-DIHYDROXYPIPERIDINE

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • metagenomes (others)
KeywordsHYDROLASE / Tim Barrel

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