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Structure paper

TitleCrystal Structure of the Homing Endonuclease I-Cvui Provides a New Template for Genome Modification
Journal, issue, pagesJ. Biol. Chem., Vol. 290, Page 28727-, Year 2015
Publish dateJul 2, 2015 (structure data deposition date)
AuthorsMolina, R. / Redondo, P. / Lopez-Mendez, B. / Villate, M. / Merino, N. / Blanco, F.J. / Valton, J. / Grizot, S. / Duchateau, P. / Prieto, J. / Montoya, G.
External linksJ. Biol. Chem. / PubMed:26363068
MethodsX-ray diffraction
Resolution2.5 - 2.6 Å
Structure data

PDB-5a72:
Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Ca
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5a74:
Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Mn
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5a77:
Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5a78:
Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA not cleaved
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • chlorella vulgaris (plant)
  • synthetic construct (others)
KeywordsHYDROLASE/DNA / HYDROLASE-DNA COMPLEX / GENE TARGETING / GENETICS / PROTEIN-DNA INTERACTION / HOMING ENDONUCLEASES

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