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TitleMechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
Journal, issue, pagesChembiochem, Vol. 16, Page 2484-2490, Year 2015
Publish dateMay 2, 2015 (structure data deposition date)
AuthorsHirayama, A. / Miyanaga, A. / Kudo, F. / Eguchi, T.
External linksChembiochem / PubMed:26426567
MethodsX-ray diffraction
Resolution2.27 - 2.4 Å
Structure data

PDB-4zm3:
Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

PDB-4zm4:
Complex structure of PctV K276R mutant with PMP and 3-dehydroshkimate
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-P3B:
(3E,4R,5R)-4,5-dihydroxy-3-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}cyclohex-1-ene-1-carboxylic acid

Source
  • streptomyces pactum (bacteria)
KeywordsTRANSFERASE / Pyridoxal 5-phosphate / Aminotransferase

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