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Structure paper

TitleStructural Basis of Stereospecificity in the Bacterial Enzymatic Cleavage of beta-Aryl Ether Bonds in Lignin.
Journal, issue, pagesJ. Biol. Chem., Vol. 291, Page 5234-5246, Year 2016
Publish dateJan 22, 2015 (structure data deposition date)
AuthorsHelmich, K.E. / Pereira, J.H. / Gall, D.L. / Heins, R.A. / McAndrew, R.P. / Bingman, C. / Deng, K. / Holland, K.C. / Noguera, D.R. / Simmons, B.A. ...Helmich, K.E. / Pereira, J.H. / Gall, D.L. / Heins, R.A. / McAndrew, R.P. / Bingman, C. / Deng, K. / Holland, K.C. / Noguera, D.R. / Simmons, B.A. / Sale, K.L. / Ralph, J. / Donohue, T.J. / Adams, P.D. / Phillips, G.N.
External linksJ. Biol. Chem. / PubMed:26637355
MethodsX-ray diffraction
Resolution1.905 - 2.593 Å
Structure data

PDB-4xt0:
Crystal Structure of Beta-etherase LigF from Sphingobium sp. strain SYK-6
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-4yam:
Crystal structure of LigE-apo form from Sphingobium sp. strain SYK-6
Method: X-RAY DIFFRACTION / Resolution: 1.905 Å

PDB-4yan:
Crystal structure of LigE in complex with glutathione (GSH) from Sphingobium sp. strain SYK-6
Method: X-RAY DIFFRACTION / Resolution: 2.593 Å

Chemicals

ChemComp-GSH:
GLUTATHIONE

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • sphingobium sp. syk-6 (bacteria)
KeywordsTRANSFERASE / beta-etherase / lignase / LigF / thioredoxin / glutathione / GST / Glutathione-S-transferase B-etherase

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