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Structure paper

TitleCrystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Journal, issue, pagesImmunity, Vol. 44, Page 21-31, Year 2016
Publish dateDec 15, 2014 (structure data deposition date)
AuthorsIrimia, A. / Sarkar, A. / Stanfield, R.L. / Wilson, I.A.
External linksImmunity / PubMed:26777395
MethodsX-ray diffraction
Resolution1.43 - 3.96 Å
Structure data

PDB-4xaw:
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41: Crystals cryoprotected with phosphatidic acid (08:0 PA)
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-4xbe:
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41: crystals cryoprotected with sphingomyelin (02:0 SM (d18:1/2:0)).
Method: X-RAY DIFFRACTION / Resolution: 1.756 Å

PDB-4xbg:
Crystal structure of human 4E10 Fab in complex with phosphatidic acid (06:0 PA): 2.73 A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

PDB-4xbp:
Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylethanolamine (06:0 PE)
Method: X-RAY DIFFRACTION / Resolution: 2.94 Å

PDB-4xc1:
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 GP41: crystals cryoprotected with sn-Glycerol 3-phosphate
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-4xc3:
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41; crystals cryoprotected with rac-glycerol 1-phosphate
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-4xcc:
Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylcholine (06:0 PC); I422 space group
Method: X-RAY DIFFRACTION / Resolution: 2.81 Å

PDB-4xce:
Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylcholine (06:0 PC); C2 space group
Method: X-RAY DIFFRACTION / Resolution: 2.93 Å

PDB-4xcf:
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41; crystals cryoprotected with phosphatidylcholine (03:0 PC)
Method: X-RAY DIFFRACTION / Resolution: 1.43 Å

PDB-4xcn:
Crystal structure of human 4E10 Fab in complex with phosphatidic acid (06:0 PA); 2.9 A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-4xcy:
Crystal structure of human 4E10 Fab in complex with phosphatidylglycerol (06:0 PG)
Method: X-RAY DIFFRACTION / Resolution: 3.96 Å

Chemicals


ChemComp, No image

ChemComp-UNL:
Unknown ligand

ChemComp-HOH:
WATER

ChemComp-44E:
(2R)-3-(phosphonooxy)propane-1,2-diyl dihexanoate / phospholipid*YM

ChemComp-GOL:
GLYCEROL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HAI:
CYCLOHEXYLAMMONIUM ION

ChemComp-G3P:
SN-GLYCEROL-3-PHOSPHATE

ChemComp-1GP:
SN-GLYCEROL-1-PHOSPHATE

ChemComp-43Y:
[(2R)-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-2-propanoyloxy-propyl] propanoate

ChemComp-44G:
(2S)-3-{[(R)-{[(2R)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-(hexanoyloxy)propyl hexanoate

Source
  • homo sapiens (human)
  • human immunodeficiency virus 1
KeywordsIMMUNE SYSTEM / HIV-1 gp41 MPER / 4E10 Fab / lipid membrane / membrane lipid / human anti gp41 HIV-1 4E10 Fab / phosphatidic acid / human 4E10 Fab anti HIV-1 gp41 / membrane lipids / Phosphatidylethanolamine / 4E10 Fab anti HIV-1 gp41 / Phosphatidylcholine / 4E10 Fab anti HIV-1 gp41 MPER / phosphatidylglycerol

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