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Structure paper

TitleDirected Divergent Evolution of a Thermostable D-Tagatose Epimerase towards Improved Activity for Two Hexose Substrates.
Journal, issue, pagesChembiochem, Vol. 16, Page 592-601, Year 2015
Publish dateApr 29, 2014 (structure data deposition date)
AuthorsBosshart, A. / Hee, C.S. / Bechtold, M. / Schirmer, T. / Panke, S.
External linksChembiochem / PubMed:25655925
MethodsX-ray diffraction
Resolution1.8 - 2.1 Å
Structure data

PDB-4pfh:
Crystal structure of engineered D-tagatose 3-epimerase PcDTE-IDF8
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4pgl:
Crystal structure of engineered D-tagatose 3-epimerase PcDTE-ILS6
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4q7i:
Crystal structure of engineered thermostable D-tagatose 3-epimerase PcDTE-Var8
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-PSJ:
D-psicose

ChemComp-FUD:
D-fructose

ChemComp-HOH:
WATER

ChemComp-SOL:
L-sorbose

ChemComp-SOE:
alpha-L-sorbopyranose

ChemComp-LTG:
L-tagatose

ChemComp-GOL:
GLYCEROL

ChemComp-IMD:
IMIDAZOLE

Source
  • pseudomonas cichorii (bacteria)
  • escherichia coli (E. coli)
KeywordsISOMERASE / epimerase / TIM-barrel

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