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Structure paper

TitleCrystal structure of the ectoine hydroxylase, a snapshot of the active site.
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 29570-29583, Year 2014
Publish dateAug 30, 2013 (structure data deposition date)
AuthorsHoppner, A. / Widderich, N. / Lenders, M. / Bremer, E. / Smits, S.H.
External linksJ. Biol. Chem. / PubMed:25172507
MethodsX-ray diffraction
Resolution2.1 - 2.64 Å
Structure data

PDB-4mhr:
Crystal structure of EctD from S. alaskensis in its apoform
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4mhu:
Crystal structure of EctD from S. alaskensis with bound Fe
Method: X-RAY DIFFRACTION / Resolution: 2.56 Å

PDB-4q5o:
Crystal structure of EctD from S. alaskensis with 2-oxoglutarate and 5-hydroxyectoine
Method: X-RAY DIFFRACTION / Resolution: 2.64 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-FE:
Unknown entry

ChemComp-D9G:
N-DODECYL-N,N-DIMETHYLGLYCINATE

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-6CS:
(4S,5S)-5-HYDROXY-2-METHYL-1,4,5,6-TETRAHYDROPYRIMIDINE-4-CARBOXYLIC ACID

Source
  • sphingopyxis alaskensis (bacteria)
  • sphingopyxis alaskensis rb2256 (bacteria)
KeywordsOXIDOREDUCTASE / 2-His-1-carboxylate facial triad cupin fold / ectoine / jelly-roll or cupin fold / ectoine hydroxylase

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