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Structure paper

TitleStructural and Functional Characterization of MppR, an Enduracididine Biosynthetic Enzyme from Streptomyces hygroscopicus: Functional Diversity in the Acetoacetate Decarboxylase-like Superfamily.
Journal, issue, pagesBiochemistry, Vol. 52, Page 4492-4506, Year 2013
Publish dateMar 13, 2013 (structure data deposition date)
AuthorsBurroughs, A.M. / Hoppe, R.W. / Goebel, N.C. / Sayyed, B.H. / Voegtline, T.J. / Schwabacher, A.W. / Zabriskie, T.M. / Silvaggi, N.R.
External linksBiochemistry / PubMed:23758195
MethodsX-ray diffraction
Resolution1.67 - 1.85 Å
Structure data

PDB-4jm3:
Enduracididine Biosynthesis Enzyme MppR with HEPES Buffer Bound
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4jmc:
Enduracididine biosynthesis enzyme MppR complexed with pyruvate
Method: X-RAY DIFFRACTION / Resolution: 1.72 Å

PDB-4jmd:
Enduracididine biosynthesis enzyme MppR complexed with the condensation product of pyruvate and imidazole 4-carboxaldehyde
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-4jme:
Enduracididine biosynthesis enzyme MppR complexed with 2-keto-enduracididine
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-PYR:
PYRUVIC ACID

ChemComp-4IC:
(3E)-4-(1H-imidazol-4-yl)but-3-enoic acid

ChemComp-ECD:
(4R)-4-(2-carboxyethyl)imidazolidin-2-iminium

Source
  • streptomyces hygroscopicus (bacteria)
KeywordsUNKNOWN FUNCTION / Acetoacetate decarboxylase-like

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