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Structure paper

TitlePLP undergoes conformational changes during the course of an enzymatic reaction.
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 70, Page 596-606, Year 2014
Publish dateJan 28, 2013 (structure data deposition date)
AuthorsNgo, H.P. / Cerqueira, N.M. / Kim, J.K. / Hong, M.K. / Fernandes, P.A. / Ramos, M.J. / Kang, L.W.
External linksActa Crystallogr. ,Sect. D / PubMed:24531493
MethodsX-ray diffraction
Resolution1.7 - 2.29 Å
Structure data

PDB-4ixs:
Native structure of xometc at ph 5.2
Method: X-RAY DIFFRACTION / Resolution: 2.29 Å

PDB-4ixz:
Native structure of cystathionine gamma lyase (XometC) from xanthomonas oryzae pv. oryzae at pH 9.0
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-4iy7:
crystal structure of cystathionine gamma lyase (XometC) from Xanthomonas oryzae pv. oryzae in complex with E-site serine, A-site external aldimine structure with serine and A-site external aldimine structure with aminoacrylate intermediates
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4iyo:
Crystal structure of cystathionine gamma lyase from Xanthomonas oryzae pv. oryzae (XometC) in complex with E-site serine, A-site serine, A-site external aldimine structure with aminoacrylate and A-site iminopropionate intermediates
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CO3:
CARBONATE ION

ChemComp-HOH:
WATER

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-SER:
SERINE

ChemComp-SO4:
SULFATE ION

ChemComp-0JO:
2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid

ChemComp-PYR:
PYRUVIC ACID

ChemComp-KOU:
(E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine

ChemComp-NAK:
AMINO-ACRYLATE

Source
  • xanthomonas oryzae pv. oryzae (bacteria)
KeywordsLYASE / PLP DEPENDENT ENZYME / XOMETC / Cys-Met metabolism PLP dependent enzyme / cystathionine gamma/beta lyase / PLP Binding / XOCGL / NATIVE / cystathionine gamma lyase / SERINE EXTERNAL SCHIFF BASE / AMINOACRYLATE EXTERNAL SCHIFF BASE / IMINOPROPIONATE / SERINE

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